Toxicity Effect of Pb(Ⅱ) on Two Diff

Toxicity Effect of Pb(Ⅱ) on Two Different Kinds of Microbes Measured by Microcalorimetry

In this work,microcalorimetric technique was used to analyze Pb(Ⅱ)toxic action on the metabolic activities of Candida humicola and Bacillus subtilis.The experimental results revealed that Pb(Ⅱ)had a stimulating effect on C.humicola and B.subtilis growth at a relatively low concentration(10.0μg·mL-1);while,C. humicola and B.subtilis were inhibited completely when the concentrations were up to 320.0 and 160.0μg· mL-1,respectively,and the rela-tionships between growth rate constant(K)and doses of Pb(Ⅱ)were approximately linear for the two microbes at certain concerntrations.At the same time.their cell dry weight and turbidity(OD600)during growth were also ob-rained.Their thermogenic cnrves of the growth coincided well with their turbidity curves,elucidating that the mi-crocalorimetdc method agreed witll the routine microbiology methods.A11 of these corroborate the validity and sensitivity of the microcalorimetric technique to investigate the toxic effect of Pb(Ⅱ)On soil microorganisms.

作 者： GAI,Nan YAO,Jun TIAN,Lin CHEN,Huilun ZHOU,Yong WANG,Fei CHEN,Yanjiao CHEN,Hai

yan CHEN,Ke TREBSE,Polonca ZARAY,Gyula 作者单位： GAI,Nan,YAO,Jun,TIAN,Lin,CHEN,Huilun,ZHOU,Yong,WANG,Fei,CHEN,Yanjiao,CHEN,Haiyan,CHEN,Ke(School of Environmental Studies &Key Laboratory of Biogeology and Environmental Geology of Chinese Ministry of Education & Sino-Hungarian Joint Laboratory of Environmental Science and Health China University of Geosciences,Wuhan,Hubei 430074,China)

TREBSE,Polonca(Laboratory for Environmental Research,University of Nova Gorica,Vipavska 13,5000 Nova Gorica,Slovenia)

ZARAY,Gyula(Department of Chemical Technology and Environmental Chemistry,E(o)tv(o)s University,H-1518 Budapest,P.O.Box 32,Hungary)

刊 名： 中国化学（英文版） SCI 英文刊名： CHINESE JOURNAL OF CHEMISTRY 年，卷(期)： 200927(3) 分类号： O6 关键词： Candida humicola Bacillus subtilis microcalorimetry lead toxic effect