染雾Expression and localization of VCX/Y 篇一
VCX/Y is a group of genes located on the X and Y chromosomes that are primarily expressed in the testes and play a crucial role in male fertility. Understanding the expression and localization patterns of VCX/Y genes is essential for unraveling their functions and potential implications in infertility.
The VCX/Y gene family consists of multiple members, including VCX, VCX2, VCX3A, and VCX3B, which are located on the X chromosome, and VCX-Y and VCX2-Y, which are located on the Y chromosome. These genes encode a family of proteins called Variable Charge, X-linked (VCX) proteins, which are highly expressed in the testes and have been suggested to be involved in spermatogenesis.
Studies have shown that the expression of VCX/Y genes is tightly regulated during different stages of spermatogenesis. In the early stages, the expression levels of VCX/Y genes are relatively low. However, as spermatogenesis progresses, the expression levels of these genes increase dramatically, reaching their peak in mature spermatozoa. This suggests that VCX/Y genes play a crucial role in the later stages of spermatogenesis and sperm maturation.
In terms of localization, VCX/Y proteins have been found to be predominantly localized to the cytoplasm of spermatocytes and round spermatids. This suggests that VCX/Y proteins may play a role in the development and maturation of spermatozoa. In addition, VCX/Y proteins have also been detected in the midpiece and tail of mature spermatozoa, suggesting that they may have a role in sperm motility.
Furthermore, studies have shown that the expression and localization of VCX/Y genes may be altered in certain conditions associated with male infertility. For example, it has been reported that the expression levels of VCX/Y genes are significantly decreased in patients with azoospermia, a condition characterized by the absence of spermatozoa in the semen. This suggests that abnormalities in VCX/Y gene expression may contribute to the pathogenesis of azoospermia.
In conclusion, the expression and localization patterns of VCX/Y genes are tightly regulated during spermatogenesis and play a crucial role in male fertility. Further research is needed to fully understand the functions of VCX/Y genes and their potential implications in infertility. Understanding the molecular mechanisms underlying VCX/Y gene expression and localization may lead to the development of novel diagnostic and therapeutic strategies for male infertility.
Expression and localization of VCX/Y 篇三
Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during s
Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromo-somes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST)fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated andthe localization of VCY protein in human testis was determined by immunohistochemistry. In the testisseminiferous epithelium, VCY proteins were highly expressed in nuclei of germ cells. Using propidium io-dide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainlylocalized in the nucleoli of COS7 cells. In addition, the colocalization for VCY and VCX-8r in COS7 cellswas also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtainedusing yeast two-hybrid system. All the information above indicates that VCX/Y protein family might beinvolved in the regulation of ribosome assembly during spermatogenesis.
作 者： SHENG WEI ZOU JIAN CHAO ZHANG Xiao Dong ZHANG Shi Ying MIAO SHU DONG ZONG QI
JIAN CHAO ZHANG,SHU DONG ZONG(National Research Institute for Family Planing, WHO Collaboration Center for Research in Human Reproduction,12 Da Hui Si,Beijing 100081, China)
刊 名：细胞研究(英文版) ISTIC SCI 英文刊名： CELL RESEARCH 年，卷(期)： 200313(3) 分类号： Q2 关键词： VCX/Y protein spermatogenesis nucleoli colocalization